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CONTENTS

Cover

Title Page

Chapter 1: Introduction to Dermatological Tests

What Equipment Will You Need?
Use and Abuse of the Practice Microscope
Stains
Using Internal and External Laboratories
Sensitivity, Specificity, and Positive and Negative Predictive Value

Chapter 2: Looking for Parasites

Introduction
Visual Inspection
Coat Brushings
Tape-Strips
Hair Plucks
Skin Scrapes
Pustules and Draining Sinus Tracts
Potassium Hydroxide Versus Liquid Paraffin
Vacuuming
Faecal Examination
Parasites in the Environment
Specificity and Sensitivity
Common Mistakes and Pitfalls
Parasite Identification Guide

Chapter 3: Hair Plucks and Trichograms

Introduction
Technique
Hair Pluck Findings and their Significance

Chapter 4: Dermoscopy

Dermoscopes
Uses of Dermoscopy

Chapter 5: Cytology

Introduction
Indications for Cytology
Cytological Techniques
Cytological Findings
Algorithmic Approach
Common Pitfalls and Mistakes

Chapter 6: Fungal and Bacterial Cultures and Identification

Introduction
Commensal and Transient Organisms
When to Perform Cultures
Obtaining Material for Culture
How to Interpret Bacterial Culture and Sensitivity Results
Common Pitfalls for Bacterial Cultures
Wood's Lamp
Dermoscopy
Fungal Culture Media
Identifying Common Fungal Pathogens
Detecting Unusual Fungal and Bacterial Organisms (e.g. Deep Fungal Infections and Mycobacteria)
Antifungal Susceptibility Testing

Chapter 7: Introduction to Histopathology

Indications for Performing a Biopsy
Considerations Before Performing a Biopsy
Biopsy Technique
Submission of the Samples
Does the Histopathology Make Sense?
The Histopathology Report
Basic Histopathological Terminology
Pattern Analysis
Hair Follicle Pathology

Chapter 8: Allergy Testing

Food Trials
Food-Allergen Serology and Patch Tests
Allergy Testing for Environmental Allergens

Chapter 9: Immune-Mediated Skin Diseases

Skin Biopsy and Histopathology
Specific Serology Tests
Anti-Nuclear Antibody Test
Coomb's Tests
Anti-Platelet Antibodies
Rheumatoid Factor
Cold Agglutinin Titres
Urinary Protein
Other Tests

Chapter 10: Endocrine and Metabolic Skin Diseases

Clinical Signs Associated with Endocrinopathies
Hair Plucks
Routine Haematology and Biochemistry
Urine Analysis
Analysis of Thyroid Function for Dogs with Suspected Hypothyroidism
Analysis of Thyroid Function for Cats with Suspected Hyperthyroidism
Tests to Confirm Hyperadrenocorticism
Sex Hormone Assays
Miscellaneous Endocrine and Metabolic Tests

Chapter 11: Infectious Diseases

Laboratory Techniques
Diagnosis of Infectious Diseases
Virology Techniques
Diagnosing Viral Diseases

Chapter 12: Diagnostic Imaging

Imaging for Patients with Ear Disease
Imaging for Patients with Endocrine Disease
Imaging for Patients with Paraneoplastic Associated Skin Disease
Other Uses for Imaging

Chapter 13: Otoscopy and Examination of the Ear

Examination of the Pinnae, Ear Canal and Tympanic Membrane
Techniques to Assess the Integrity of the TM
Sample Collection for Cytology
Interpretation of Otic Cytology
Bacterial Culture and Antimicrobial Sensitivity Testing
Myringotomy and Evaluation of the Middle Ear
BAER Testing
Biopsy

Chapter 14: Which Test to Choose When

Introduction
Pruritus
Otitis
Alopecia
Scaling and Crusting Dermatoses
Pigmentary Changes
Macules, Papules and Pustules
Ulcers and Erosions
Importance of Making the Correct Diagnosis and Specialist Referral

Chapter 15: Genetic Tests for Skin Diseases

Patterns of Inheritance
Genetic Tests
Breeding Advice

References

Index

End User License Agreement

List of Illustrations

Figure 1.1

Figure 1.2

Figure 1.3

Figure 1.4

Figure 1.5

Figure 1.6

Figure 1.7

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Figure 1.9

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Figure 1.11

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Figure 2.1

Figure 2.2

Figure 2.3

Figure 2.4

Figure 2.5

Figure 2.6

Figure 2.7

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Figure 2.10

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Figure 2.12

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Figure 2.19

Figure 3.1

Figure 3.2

Figure 3.3

Figure 3.4

Figure 3.5

Figure 3.6

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Figure 3.10

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Figure 3.12

Figure 3.13

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Figure 3.15

Figure 4.1

Figure 4.2

Figure 4.3

Figure 5.1

Figure 5.2

Figure 5.3

Figure 5.4

Figure 5.5

Figure 5.6

Figure 5.7

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Figure 5.10

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Figure 5.14

Figure 5.15

Figure 5.16

Figure 6.1

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Figure 6.10

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Figure 6.17

Figure 7.1

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Figure 8.1

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Figure 9.1

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Figure 10.1

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Figure 10.18

Figure 10.19

Figure 11.1

Figure 11.2

Figure 11.3

Figure 11.4

Figure 11.5

Figure 11.6

Figure 11.7

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Figure 11.9

Figure 11.10

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Figure 12.1

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Figure 12.13

Figure 12.14

Figure 13.1

Figure 13.2

Figure 13.3

Figure 13.4

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Figure 13.6

Figure 13.7

Figure 13.8

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Figure 13.10

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Figure 13.13

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Figure 13.16

Figure 13.17

Figure 13.18

Figure 13.19

Figure 13.20

Figure 13.21

Figure 13.22

Figure 13.23

Figure 14.1

Figure 14.2

Figure 14.3

Figure 14.4

Figure 14.5

Figure 14.6

Figure 14.7

Figure 14.8

Figure 15.1

Figure 15.2

Figure 15.3

Figure 15.4

This edition first published 2017

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Library of Congress Cataloging-in-Publication Data

Names: Neuber, Ariane, 1970- author. | Nuttall, Tim, 1967- author.

Title: Diagnostic techniques in veterinary dermatology : a manual of diagnostic techniques / Ariane Neuber, Tim Nuttall.

Description: Hoboken, NJ, USA : Wiley-Blackwell, 2017. | Includes bibliographical references and index. |

Identifiers: LCCN 2017005977 (print) | LCCN 2017015845 (ebook) | ISBN 9781119233046 (Adobe PDF) | ISBN 9781119233060 (ePub) | ISBN 9781405139489 (paperback)

Subjects: LCSH: Veterinary dermatology. | MESH: Skin Diseases–veterinary | Dog Diseases–diagnosis | Cat Diseases–diagnosis

Classification: LCC SF901 (ebook) | LCC SF901 .N48 2017 (print) | NLM SF 992.S55 | DDC 636.089/65–dc23

LC record available at https://lccn.loc.gov/2017005977

Cover images: courtesy of the authors